Investigating the function of the cysteine rich Receptor Kinase CRK 10

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Investigating the function of the cysteine rich Receptor Kinase CRK 10

In an Arabidopsis EMS mutagenesis screen for cis-jasmone signalling mutants, a mutant was isolated showing a dwarfed phenotype. Further analysis revealed that in this mutant the development of the vasculature in the root is impaired showing collapsed vessels and aberrant lignification patches. Whether this is responsible for the dwarfed phenotype remains to be proven. The gene responsible for the mutation was putatively identified as a cysteine-rich receptor kinase (CRK10) which so far remains uncharacterised. The EMS mutation in the receptor kinase generated an Alanine to Threonine substitution in the active kinase domain of the receptor. We postulate that the substitution of Alanine by Threonine has introduced a potential auto-phosphorylation site in the active kinase domain which could lead to the activation of the kinase in the absence of its ligand. Proving this hypothesis will be a major part of the project besides an in depth analysis of the mutant.

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